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Original Articles
TEM and SHV Genes in Klebsiella pneumoniae Isolated from Cockroaches and Their Antimicrobial Resistance Pattern
Abbas Doosti, Mohammad Pourabbas, Asghar Arshi, Mohammad Chehelgerdi, Hamidreza Kabiri
Osong Public Health Res Perspect. 2015;6(1):3-8.   Published online February 28, 2015
DOI: https://doi.org/10.1016/j.phrp.2014.10.011
  • 3,001 View
  • 47 Download
  • 14 Crossref
AbstractAbstract PDF
Objectives
Klebsiella pneumoniae is a gram-negative rod bacterium, a known cause of community-acquired bacterial pneumonia and is an important hospital-acquired pathogen that causes severe morbidity and mortality. The aim of this study was to identify the TEM and SHV genes in K. pneumoniae isolated from cockroaches obtained from hospitals.
Methods
In this study, 250 cockroaches were collected from different hospitals in the province of Chaharmahal Va Bakhtiari, which is located in southwest Iran. The samples were examined for the presence of K. pneumoniae by plating onto a combination of culture media, and the antimicrobial susceptibility patterns of isolated K. pneumoniae from samples were evaluated using the disk diffusion test. In addition, from the culture, genomic bacterial DNA was extracted, and sequence-specific targets (TEM and SHV genes) were amplified using the polymerase chain reaction (PCR) method.
Results
Out of 250 cockroach samples collected from various hospitals, 179 samples (71.60%) were positive for K. pneumoniae. PCR reaction was performed using specific oligonucleotide primers (TEM-F, TEM-R and SHV-F, SHV-R) for the amplification of each gene, and amplified products were visualized on 1% agarose gel electrophoresis. Of all the specimens amplified by PCR in this research, 32 samples (17.87%) were positive for TEM and 15 samples (8.37%) were positive for SHV.
Conclusion
Detection of TEM and SHV genes using molecular methods and their pattern of antimicrobial resistance can provide useful information about the epidemiology of and risk factors associated with K. pneumoniae infection.

Citations

Citations to this article as recorded by  
  • Novel adjuvant nano-vaccine induced immune response against Acinetobacter baumannii
    Tohid Piri-Gharaghie, Abbas Doosti, Seyed Abbas Mirzaei
    AMB Express.2023;[Epub]     CrossRef
  • Virulence factors, antibiotic resistance patterns, and molecular types of clinical isolates of Klebsiella Pneumoniae
    Mitra Ahmadi, Reza Ranjbar, Payam Behzadi, Taher Mohammadian
    Expert Review of Anti-infective Therapy.2022; 20(3): 463.     CrossRef
  • Detection of Klebsiella pneumoniae antibiotic-resistant genes: An impending source of multidrug resistance dissemination through raw food
    Kashaf Junaid, Hasan Ejaz, Sonia Younas, Awadh Alanazi, Humaira Yasmeen, Abdul Rehman
    Saudi Journal of Biological Sciences.2022; 29(5): 3347.     CrossRef
  • First Report of Extended-Spectrum β-Lactamase ( bla CTX-M1 ) and Colistin Resistance Gene mcr-1 in E. coli of Lineage ST648 from Cockroaches in Tunisia
    Sarrah Landolsi, Rachid Selmi, Linda Hadjadj, Asma Ben Haj Yahia, Kaouther Ben Romdhane, Lilia Messadi, Jean Marc Rolain, Courtney J. Robinson
    Microbiology Spectrum.2022;[Epub]     CrossRef
  • Phenotypic and genotypic detection of extended spectrum beta lactamase enzyme in Klebsiella pneumoniae
    Aso Bakr Mohammed, Khanda Abdullateef Anwar, Monica Cartelle Gestal
    PLOS ONE.2022; 17(9): e0267221.     CrossRef
  • Virulence factors and antibiotic resistance properties of Streptococcus species isolated from hospital cockroaches
    Mohammad Chehelgerdi, Reza Ranjbar
    3 Biotech.2021;[Epub]     CrossRef
  • Putative novel B-cell vaccine candidates identified by reverse vaccinology and genomics approaches to control Acinetobacter baumannii serotypes
    Sheida Beiranvand, Abbas Doosti, Seyed Abbas Mirzaei
    Infection, Genetics and Evolution.2021; 96: 105138.     CrossRef
  • Molecular epidemiology of Carbapenem-resistant Klebsiella pneumoniae in a paediatric hospital in China
    Bingjie Wang, Fen Pan, Chun Wang, Wantong Zhao, Yan Sun, Tiandong Zhang, Yingying Shi, Hong Zhang
    International Journal of Infectious Diseases.2020; 93: 311.     CrossRef
  • Fecal Carriage and Molecular Epidemiology of Carbapenem-Resistant Enterobacteriaceae from Inpatient Children in a Pediatric Hospital of Shanghai


    Qi Xu, Fen Pan, Yan Sun, Chun Wang, Yingying Shi, Tiandong Zhang, Fangyuan Yu, Hong Zhang
    Infection and Drug Resistance.2020; Volume 13: 4405.     CrossRef
  • Contamination of Cockroaches (Insecta: Blattaria) by Medically Important Bacteriae: A Systematic Review and Meta-analysis
    Hassan Nasirian, Michael Rust
    Journal of Medical Entomology.2019; 56(6): 1534.     CrossRef
  • Competition assays between ESBL-producing E. coli and K. pneumoniae isolates collected from Lebanese elderly: An additional cost on fitness
    Nourhane Hafza, Caren Challita, Iman Dandachi, Mounir Bousaab, Elias Dahdouh, Ziad Daoud
    Journal of Infection and Public Health.2018; 11(3): 393.     CrossRef
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    Joel M. Gichumbi, Holger B. Friedrich, Bernard Omondi, Kovashnee Naicker, Moganavelli Singh, Hafizah Y. Chenia
    Journal of Coordination Chemistry.2018; 71(2): 342.     CrossRef
  • Relationship between β-lactamase production and resistance phenotype in Klebsiella pneumoniae strains
    Jeongjin Kim, Tian Ding, Juhee Ahn
    FEMS Microbiology Letters.2017;[Epub]     CrossRef
  • First Report of German Cockroaches (Blattella germanica) as Reservoirs of CTX-M-15 Extended-Spectrum-β-Lactamase- and OXA-48 Carbapenemase-Producing Enterobacteriaceae in Batna University Hospital, Algeria
    Lotfi Loucif, Djamila Gacemi-Kirane, Zineb Cherak, Naima Chamlal, Nadia Grainat, Jean-Marc Rolain
    Antimicrobial Agents and Chemotherapy.2016; 60(10): 6377.     CrossRef
Molecular Investigation of Quinolone Resistance of Quinolone Resistance-Determining Region in Streptococcus pneumoniae Strains Isolated from Iran Using Polymerase Chain Reaction–Restriction Fragment Length Polymorphism Method
Mohammad Kargar, Fataneh Moein Jahromi, Abbas Doosti, Somayeh Handali
Osong Public Health Res Perspect. 2014;5(5):245-250.   Published online October 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.08.010
  • 2,858 View
  • 22 Download
  • 2 Crossref
AbstractAbstract PDF
Objectives
The resistance of Streptococcus pneumoniae to the recently available antibiotic treatment has been a growing problem. The aim of the study was to determine the quinolone-resistant strains and detect the presence of mutations in the quinolone resistance-determining regions of the gyrA, parE, and parC genes.
Methods
In this study, for the first time in Iran, the polymerase chain reaction–restriction fragment length polymorphism (PCR-RFLP) method was used to investigate the presence of mutations at quinolone resistance-determining regions of topoisomerase IV and DNA gyrase on 82 S. pneumoniae strains, among them 45 clinical samples were from patients and 37 from healthy carriers (control group).
Results
In clinical samples, 34 (75.56%) strains contained mutations in the parC gene, 31 (68.89%) carried mutations in the gyrA gene, and 14 (31.11%) had parE gene mutations. Antibiotic susceptibility test was performed using the CLSI (Clinical and Laboratory Standards Institute) criteria on three different generations of quinolone family, with nalidixic acid (82.22%) showing the highest resistance and levofloxacin (42.22%) the least resistance.
Conclusion

Results
indicated that there is a significant correlation between quinolone resistance development and mutations in the parE gene as well as in the parC and gyrA genes.

Citations

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  • DNA-based detection of pork content in food
    Muflihah, Ari Hardianto, Pintaka Kusumaningtyas, Sulistyo Prabowo, Yeni Wahyuni Hartati
    Heliyon.2023; 9(3): e14418.     CrossRef
  • Mutant selection window of four quinolone antibiotics against clinical isolates of Streptococcus pneumoniae , Haemophilus influenzae and Moraxella catarrhalis
    Hajime Nakai, Takumi Sato, Takashi Uno, Emiko Furukawa, Masato Kawamura, Hiroshi Takahashi, Akira Watanabe, Shigeru Fujimura
    Journal of Infection and Chemotherapy.2018; 24(2): 83.     CrossRef
High Prevalence of Class 1 to 3 Integrons Among Multidrug-Resistant Diarrheagenic Escherichia coli in Southwest of Iran
Mohammad Kargar, Zahra Mohammadalipour, Abbas Doosti, Shahrokh Lorzadeh, Alireza Japoni-Nejad
Osong Public Health Res Perspect. 2014;5(4):193-198.   Published online August 31, 2014
DOI: https://doi.org/10.1016/j.phrp.2014.06.003
  • 3,205 View
  • 25 Download
  • 44 Crossref
AbstractAbstract PDF
Objectives
Horizontal transfer of integrons is one of the important factors that can contribute to the occurrence of multidrug-resistant (MDR) bacteria. This study aimed to determine the prevalence of integrons among MDR Escherichia coli strains isolated from stool specimens and investigate the associations between the existence of integrons and MDR properties in the southwest of Iran.
Methods
There were 164 E. coli strains isolated from January 2012 to June 2012. Fecal specimens identified as E. coli by the conventional methods. Subsequently the antibiotic resistance was assessed using Clinical and Laboratory Standard Institute criteria. The presence of class 1–3 integrons and embedded gene cassettes was verified using specific primers by multiplex polymerase chain reaction assay.
Results
Among a total of 164 studied samples, 69 (42.07%) isolates were multidrug resistant. Class 1 and class 2 integrons were present in 78.26% and 76.81% MDR isolates, respectively. For the first time in Iran, class 3 integron was observed in 26.09% MDR isolates. Significant correlations were identified between: class 1 integron and resistance to amikacin, gentamicin, chloramphenicol, ampicillin, tetracycline, nalidixic acid, and co-trimoxazole; class 2 integron and resistance to aminoglycosides, co-trimoxazole, cefalexin, ampicillin, and chloramphenicol; and class 3 integron and resistance to gentamicin, kanamycin, and streptomycin.
Conclusion
Our results indicate that integrons are common among MDR isolates and they can be used as a marker for the identification of MDR isolates. Therefore, due to the possibility of a widespread outbreak of MDR isolates, molecular surveillance and sequencing of the integrons in other parts of the country is recommended.

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PHRP : Osong Public Health and Research Perspectives